As a characteristic of a long time exploitation and industry cluster, Daxie Island has been studied in the ecological response of exploitation in macrozoobenthos. Comparing with similar biotope in other adjacent islands with lower exploitation, we have analyzed the species composition, community structure, and amount distribution of marine macrozoobenthos in different biotope (cay, tide flat and sea area) in Daxie Island. The result showed that: (1) There were a higher density of Ostreidae and Balanidae and a lower biodiversity in the cay sampling section of Daxie Island. (2) No significant difference was found in the macrozoobenthos community among the tide plat sampling section in Daxie Island. (3) There was a lower macrozoobenthos species number, biodiversity, and a higher density of Sternaspis scutata and Capitella capitata than those in the sea area beside Daxie Island. Moreover, a significant difference was found in the macrozoobenthos community between the sea area of Daxie Island and the adjacent islands. (4) The biologic assessment showed more serious disturbance presented in sea area beside Daxie Island. In additional, some solutions for conservation, recovery biotope, and biological diversity were put forward.