The experiments were conducted by planting in the field and pot-culturing of 2 year-old Mongolian Pine (Pinus sylvstris var. mongolica) seedlings processed with Pt mycorrhizal agents, ABT rooting powder, HRC water-absorbing agent and GRR plant development and growth regulator. The effect and function of the biological agents on survival rate of seedlings were studied by measuring height growth, biomass, roots, net photosynthetic rates, transpiration rates and water use efficiency. The results were as follows: (1) Pt3 (No. 3 mycorrhizal agent), ABT (ABT rooting powder) and HRC (HRC water-absorbing agent) had significantly increased survival rates of seedlings, which were 29.3%, 23.6% and 94% higher than that of controlled seedlings, respectively. But the GRR (GRR plant development and growth regulator) and Pt1 (No. 1 mycorrhizal agent) had no significant effects on the survival rate of seedlings comparing that of untreated seedlings; (2) Pt3 and ABT had enhanced the growth of the fine root(<2mm), which were 34.72 and 15.18 cm longer than that of controlled seedlings; the regression analysis had showed that fine roots quantity was positively related to seedling survival rate, which indicated that enhancing of survival rate of seedlings was achieved by increasing fine roots; (3) The heights of the average individuals with Pt3, ABT and HRC were 2.2, 1.5 and 1.1 cm, respectively higher than that of untreated seedlings. The dry biological mass of average seedling was 1.73, 0.72 and 0.33g heavier than that of average untreated seedling. Lengths of needles, the single leaf area and leaf quantity of a single seedling were 1.54cm and 0096cm, 0.188 and 0.116cm2 and 31 and 23 bunches, respectively, higher than those of untreated seedlings, Pt1 and GRR have no effects on the height and dry biological mass of average seedling; (4) Pt and ABT also improved effectively leaf physiological mechanism of seedlings and increased chlorophyll and photosynthetic capability and water use efficiency, and the other biological agents had no effects on the seedlings. The biological agents enhanced the survival rate of Mongolian pine (Pinus sylvestris var. mongolica) in the sandy land by increasing the growth and physiological characteristics of the seedling.