To use and conserve reasonably natural enemies for comprehensive control of Bemisia tabaci, in this present work, the quality, time and spatial framework of Bemisia tabaci and its predatory natural enemies was further studied using grey system analysis, ecological niche analysis and aggregated-intensity index analysis of spatial patterns. The synthetic ranking result indicated that the order of main natural enemies of Bemisia tabaci adults was Clubiona japonicola, Therdiong octomaculatum, Erigonidium graminicola and Orius minutes. The numbers of Bemisia tabaci adults during spring-summer and autumn-winter season in pepper fields was compared using T-test (T= 7.9063, t>t0.01 = 2.85), which showed a highly significant difference. In addition, there were also highly significant differences in the numbers of predatory natural enemies, i.e. Therdiong octomaculatum(T= 4.8553, t>t0.01=2.85), Harmonia axyridis(T= 3.1780, t>t0.01=285), Clubiona japonicola(T= 3.6550, t>t0.01=2.85), Erigonidium graminicola ( T=4.7893, t>t0.01=2.85), Propylaea japonica(T= 3.2325, t>t0.01=2.85), and Tetragnatha maxillosa(T=2.1009, t>t0.05=2.09) between the two seasons in pepper fields. The further T test showed that the numbers of Bemisia tabaci adults population in autumn-winter season existed significant difference (T=2.4564, t>t0.05=2.09), while no significant difference was found in spring-summer season (T=1.4628, t