A purge & trap injector (PTI)-GC/MS was used to detect volatiles in Platycladus orientalis L. phloem. Data were compared with the thermo-desorption & cold trap (TCT)-GC/MS method. Results showed that sample quantity of 02g and purge time of 5 minutes were adequate for separation and identification, and that backflash time of 2 minutes was sufficient for effectively removing the water vapor from the air and deicing the trap. Nine monoterpenes and three sesquiterpenes were identified by using both methods. Compared with TCT-GC/MS, PTI-GC/MS has the advantages of less quantity of samples required, no interference by absorbent, no thermal decomposition, and more sensitive to the monoterpenes, making it effective for rapid and accurate determination of conifer volatiles.