Effects of different sediment nutrients on the growth and photosynthesis fluorescence characteristics of Hydrilla verticillata (L.f)Royle have been investigated in laboratory. It showed that the best environment of Hydrilla verticillata (L.f)Royle:TN/TP of overlying water was 20-50,the range of TP was 0.03-0.05 mg/L, relative ratio of phosphor was 033 mg/(m2?d). Hydrilla verticillata (L.f)Royle showed high adjustability of environment, photosynthesis fluorescence parameter Fv/Fm,qP,ETRmax increased obviously. qN increased to dissipate superfluous light protect itself when the environment was not the most suitable one.