Abstract:Vertical slab polyacrylamide gel electrophoresis was used to examine the genetic variability,mating system and to infer the level of inbreeding depression of a Cyclobalanopsis glauca population in Diaoqiao,Huangshan. Moderate genetic variation was found in the population which polymorphic loci percentage and expected heterozygosity were 50% and 0.1814, respectively. Three polymorphic loci, POD 2,EST 1 and EST 2, were used to estimated gene frequencies and outcrossing rate utilizing MLT program.Differ...