Seismic fault belt is a main passageway of various kinds of gas and geochemistry elements, where are important zone for matters, energy and information transfer between lithosphere and atmosphere. Microbes living in the belt effected intensively by the hydrogeochemical changes caused by earth crust movement.The 10th spring of Urumq originates from Bogeda mountain of Tianshan mountain range, and the groundwater runoff in seismic fault belt. The spring water contains various geochemistry elements, such as sulfide, methane, hydrogen, radon, sulfide, methane, carbon dioxide, helium, fluorine and hydrarg et al, many of which may potentially act as electron donors capable of supporting chemolithotrophy-based primary production. In order to reveal the impact of hydrogeochemical changes to bacterial community in seismic fault belt spring water, thirty-one samples of spring water collected from October 12th to November 11th and their corresponding geochemial parameters were determined. Of them, ten samples were used for bacterial dynamic changing monitoring. The planktonic communities were collected by microporous membrane filtration and then total DNA were extracted by SDS-enzymatic disruption method. The V3 region of the 16S rDNA gene of those samples was analyzed by PCR-DGGE (Denaturing Gradient Gel Electrophoresis) and different bands were excised for sequencing. The DGGE fingerprints combined with hydrogeochamical parameters were analyzed by CCA (Canonical correspondence analysis). The results showed that B16 (Uncultured bacterium) and epsilon proteobacterium were positive correlate to Fluorine fluctuation and clustered as group one and two, respectively; B1 (Flavobacterium), Pseudomonas aeruginosa, B10 (Uncultured bacterium) and Staphylococcus saprophyticus were positive correlate to sulfide change; Thiomicrospira arctica, B3 (Flavobacterium) and Staphylococcus arlettae were positive correlated to hydrogen content. The result indicated that bacterial communities in seismic fault belt spring water could response sensitively to hydrogeochemical parameter changes, and this may provide a novel means for crust movement monitoring and earthquake forecast.