We examined interspecific association of 12 tree species, 20 shrub species, and 21 herbacious species of the natural secondary forests at Ziwuling Bbased on the use of variance ratio (VR), x2 test and 2×2 contingency table, the overall interspecific association of the main 12 tree species, 20 shrub species and 21 herb species of natural secondary forest at Ziwuling were studied. There were positive associations among tree species and among shrub species. No relationship was detected among the herb species.The result showed that the interspecific association in tree layer or shrub layer were significantly positive while not significantly positive in herb layer. Among the tree species, seven pairs of species including The primary species in the tree layer were independent from each other, whereas seven pairs of species like Quercus wutaishanica and Pinus tabulaefomis, P. tabulaefomis and Acer ginnal, A. ginnal and Betula platyphylla, Pyrus betulaefolia and Q. wutaishanica, P. tabulaefomis, A. ginnal, and B. platyphylla showedhad positive associations; the number of species pairs having of positive association was twice two times that showing of negative association. in number. In shrub layer, three pairs of species includingsuch as Celastrus orbiculatus and Rubus corchorifolius, Cotoneaster maltiflorus and Cornus alba, and Lespedeza floribunda and Syringa oblate had highlyextremely significantly positive association;, and four pairs of shrub species including like Spiraea frischiana and Vitis amurensis, Rhamuns utilis and Euonymus phellomanes, C. orbiculatus and Vitis amurensis, and C. orbiculatus and Lonicera ferdinandii had were in significantly positive association. The number of pairs of species having The species pairs of positive association were approximately equivalent to those having of that of negative association. The associations among In herbaceous species were weak and inconsistant. layer, the interapecific association showed the dispersive feature, Twenty-one21 species pairs had nowithout interspecific associations, and the The ratio of species pairs of having positive association to species pairs of having negative association was 3∶2. The The several indicators applied together were proved proven to be more rational for describing the interspecific association.