The multidiscipline integrated investigations on the spawning ground of the southern waters to the Shandong Peninsula were carried out from May to July during 2000-2004 by R/V Beidou. Two drift experiments were deployed at 35°01.9′N、120°32.9′E for 25 hours from 17 to 18 and at 35°03.3′N、120°26.4′E for 64 hours from 27 to 30, June of 2003. The main goal of this survey is to get the amount of anchovy eggs, the eggs’ vertical distribution and the water temperature profile. It is shown that the variation of water temperature profile, the water mixing intensity and the convergence/divergence processes could have significant influences to the amount of anchovy eggs and their distribution in the water column. The variation of the depth of thermocline could induce the water vertical exchange, horizontal advection（convergence/divergence） and the mass exchange across the thermocline. When the thermocline became shallower, the upper mixing layer turned to thinner. The upward entrainments across the thermocline could mix the eggs of the lower layer into the thermocline and the upper mixing layer respectively. At the same time, the surface water divergence made the amount and density of anchovy eggs in the lower mixing layer, the thermocline and the upper mixing layer decrease which cause the amount of eggs less in the whole water column. On the opposite, when thermocline deepening the upper mixing layer became thicker. The downward entrainment could transport the eggs of the upper layer into the thermocline and the lower mixing layer respectively. Meanwhile the surface water convergence caused the increasing of the amount and density of anchovy eggs in the three layers and the whole water column. The amount and density of eggs in the thermocline varied much larger than that of the two mixing layers. The existence of the thermocline, the variation and its extent of the thermocline depth could dominate the variation of the amount and density of eggs. These may cause the huge change of the eggs′ vertical distribution.