Basing on the analysis of fish spawning ground′s topography characteristics, the paper pointed out that flow-energy loss caused by turbulence of special riverway physiognomy is one of the important forming causes of fish spawning sites. Deduction of unsteady open channel flow equations which considers the special flow-energy loss was carried out. The special flow-energy loss deduced by the equation can be expressed by turbulent kinetic energy. Take Chinese sturgeon for example, the turbulent kinetic energy distributions were calculated on the basis of 3-D numerical simulation to the spawning site and then we discussed the relation between Chinese sturgeon spawning behavior and turbulent kinetic energy. The results show that, because of the turbulence, the flow-energy loss of spawning regions is much higher than that of non-spawning regions. This paper could provide reference for protecting the hydraulic environment of Chinese sturgeon and other fishes.