Canonical correlation analysis was conducted on the relations between contents of 4 isomers of megastigmatrienone in flue-cured tobacco leaves and soil physical properties and nutrient conditions. 363 soil samples and its corresponding 363 flue-cured tobacco samples were collected from 12 cities in Henan Province in 2004. Soil samples were collected at 0 to 20cm depth in field. Tobacco samples were collected from 8 to 12 leaf position. Results showed that the contents of megastigmatrienone 2 and megastigmatrienone 4 fell with the increase of pH（5.15 to 8.30）, calcium, magnesium, iron, aluminium and with the decrease of sodium. The contents of megastigmatrienone 1 increased but megastigmatrienone 2 fell with the heightening of soil organic matter. The contents of megastigmatrienone 2 and megastigmatrienone 4 were improved by the boosting of the soil particles ratio between 0.05mm and 0.001mm.