Black carbon aerosol is a serious environment problem because it raised global warming as increase rapidly the CO2 concentration and atmospheric radiation, and polluted air quality, as well as impacted human health. With the increasing of forest area and biomass due to aforestation and reforestation in subtropical China, forest fire frequency becomes more high and it make big contribution to black carbon. It is very important to estimate the amount of black carbon released from forest fire in regional scale, however, there are paying less attention to it in most area of world. Based on statistical data of forest fire and the biomass of forest types in Zhejiang Province from 1991 to 2006 year, this paper reported the estimation of biomass burning from forest fire every year of Zhejiang. Meanwhile, the annually amount of black carbon released from forest fires during 1991 to 2006 year were measured using emission factors The amount of black carbon released from forest fires from 1995 to 2006 year are from 38.4 t/yr to 97.2 t/yr, The proportion of black carbon released by forest fire are 0.12% and 0.17% of whole black carbon emission in Zhejiang province in 1995 and 2006 respectively, they influence seriously air quality in the regional scale.