Based upon over ten years of investigation and species identification, 204 species of spiders belonging to 26 families in the tea plant growing areas in Guizhou Province are recorded in the current paper. The dominant spider groups include Araneidae (50 species), Salticidae (25 species), Tetragnathidae （20 species）, Thomisidae （14 species）, as well as Gnaphosidae （13 species）, which account for 60% of the total species richness. There were 64 province-wide distributed species (31% of the total species), including Larinioides cornutus, Neoscona doenitzi, Erigonidium graminicolum, Oedothorax insecticeps, Misumenops tricuspidatus, Xysticus ephippiatus, Jotus difficilis, Marpissa magister, Argiope amoena, Nephila clavata, Coleosoma octomaculatum, Pardosa astrigera, Clubiona japonicola, Oxyopes sertatus, Agelena difficilis, Pardosa tschekiangensis, Silerella vittata, Plexippus paykulli, Neoscona theisi and Tetragnatha japonica. According to the animal geographical fauna of China, a large part of Guizhou Province falls into the west mountain/plateau subregion of the central China region, while only a small part of the west Guizhou resides in the mountain subregion of the southwest China region. Based on the tea growing pattern, the spider fauna is divided into the following five sections: upland/hill section in the central Guizhou (Ⅰ), low mountain/hill and basin section in the east Guizhou (Ⅱ), low mountain and river valley section in the south Guizhou (Ⅲ), middle mountain/canyon section in the north Guizhou (Ⅳ), and plateau/middle mountain section in the west Guizhou (Ⅴ). Within section Ⅰ, 137 species belonging to 21 families were recorded, which accounts for 67％of the total species richness. There were 37 dominant species that accounts for 27% of the total species in section Ⅰ, which include Larinioides cornutus, Neoscona doenitzi, Neoscona adianta, Neoscona scylla and Argiope amoena and others. Following 13 species, Cyclosa monticola, Cyclosa informis, Cyrtarachne inaequalis, Neoscona xishanensis, Neoscona minoriscylla, Singa pygmaea， Bathyphantes tongluensis, Erigonc koshiensis, Lycosa phipsoni, Phrurolithus sinicus, Argyrodes fissifroms, Xysticus guizhou and Xysticus pseudoblitea were section-specific spider species found in section I. Within section Ⅱ, 185 species from 26 families were identified ( 91％of the total species richness), of which 31 species such as Larinioides cornutus, Clubiona japonicola, Clubiona deletrix, Oxyopes sertatus, Misumenops tricuspidatu, and others were considered as the dominant species accounting for 17% of the total species richness in this section. There were 58 section-specific species, including Uloborus sinensis, Nephila clavata, Pachygnatha quadrimaculata, Chrysso venusta, and Heptatheal jianganensis. Within section Ⅲ , 92 species from 15 families (45％ of the total species) were detected, among which 18 species in this section were considered as the dominant species including Larinioides cornutus, Erigonidium graminicolum, Oedothorax insecticeps, Gnathonarium gibberum, and Coleosoma octomaculatum consisting of accounted for 20% of the total species richness. Cyclosa vallata, Neoscona elliptica, Tylorida ventralis and Pachygnatha fengzhen were section-Ⅲ specific. In region Ⅳ, 76 species from 15 families (37％of the total species) were identified, among which 18 species including Larinioides cornutus, Agelena difficilis, Jotus difficilis, Marpissa magiste, and Plexippus paykulli were determined to be the dominant species (24% of the total species richness in section Ⅳ). Within section Ⅴ, there were 64 species belonging to 14 families were detected that occupied 31％of the total species richness, among which 15 dominant species such as Larinioides cornutus, Neoscona doenitzi, Agelena difficilis, Jotus difficilis, and Phintella melloteei etc were recognized (23% of the total species richness). There were no section-specific species in sections Ⅳ and Ⅴ. The precipitation, temperature and sunlight conditions in section Ⅲ were quite favorable for spiders; however, it was a newly cultivated tea plant growing area. Therefore, it resulted in a lower species richness than in section Ⅰ or Ⅱ. From the high and cold mountains in northwestern Guizhou to the low hills in eastern Guizhou, the altitude descends; also the climate changes from the north subtropical zone to the middle subtropical zone. As rainfall, sunlight and temperatures ascend, the abundance of tea plants and other agricultural or forest crops increase; hence, the spider species richness in the tea gardens abounds.