Urban Ecological Environmental Quality(UEQ) Assessment is the main issues of Urban ecology, and provide basis for urban region planning and ecological management. In this article, we have collected ten ecological quality assessment index based on the aspects of bio-geophysical, ecological functions, and urban environmental qualities in urban ecosystem. i.e. ecological service index, public green land per person, biodiversity index, index on infrastructure for non-industrial use etc. UEQ index can be calculated using the sum of the weighted single index. At last we select seven cities like as qindao,shanghai,changchun and others cities which have a typical environment and imporment cities as an example to examine the method we addressed，the assessmemt results have said that Qindao city have a good ecological environment quality, but Wulumuqi city is general. This results have proved this method can be used to city planning,city ecological environment repairing and city ecological environment management.