Development and reproduction of two whitefly species, Bemisia tabaci (Gennadius) biotype B and Trialeurodes vaporariorum (Westwood) were studied on three tobacco varieties (CF-965, NC-89 and NC-82) at 26℃, and at 20, 26 and 32℃ on the variety CF-965. Developmental times of immature stages of each of the two whitefly species were not different on the three tobacco varieties, whereas those of T. vaporariorum were 1.32, 2.81 and 2.04d longer than those of B. tabaci on CF-965, NC-89 and NC-82, respectively. Survival rates of immature stages of two species on CF-965 were the highest or higher compared with those on the other varieties. B. tabaci survived more on CF-965 (54.19%) than on NC-89 (23.47%) and NC-82 (19.93%), and T. vaporariorum survived the most on CF-965（31.99％）, followed by that on NC-82 (20.01%) and that on NC-89, the lowest (15.75%). The number of eggs deposited by B. tabaci was more on CF-965 and NC-89 than that and similar on NC-82 to that by T. vaporariorum. It suggested that adaptability of B. tabaci to CF-965 and NC-89 was stronger than that of T. vaporariorum. Developmental times of immature stages of both species were the longest at 20℃ and the shortest at 32℃, and the intermediate at 26℃. Survival rates of B. tabaci immatures reached 54.19 and 53.29% at 26 and 32℃, respectively, which were higher than 20.96% at 20℃. In contract, only 1166% of T. vaporariorum immatures survived at 32℃, which was lower than 31.34 and 31.99% at 20 and 26℃, respectively. The number of eggs deposited by T. vaporariorum was more than that deposited by B. tabaci at 20℃ and was less than that at 26℃. At 32℃, T. vaporariorum did not deposit eggs any more, but each female of B. tabaci still deposited about 21.06 eggs, which indicated that B. tabaci had better adaptability than T. vaporariorum under hot condition. We should pay highly attention to the occurrence of B. tabaci in the tobacco fields in Shandong province because the average temperature at the middle growth period of tobacco is around 22-30℃ that is suitable for the occurrence of B. tabaci.