The Oxytropis glabra DC. is an important poisonous plant species in Inner Mongolia steppe. In this research the endophytic fungi from O.glabra DC. were studied both by microbiological and molecular biological techniques. The results showed as follows: The in vitro cultured endophytic fungi displayed round, raised, with uniform margin and radial growth colonies which grew slowly. Later a black brown pigment was secreted in each colony. The conidia were near cylindraceous with dark transverse septa thicker than conidial walls. The septa numbers varied from 1 to 5. The sequences of 5.8S rDNA/ITS from 10 strains of O.glabra DC. were highly similar to that of Embellisia sp.L12, which indicated the endophytes might belong to Embellisia.