Tolerance values，which represent the relative capacities of survival and reproduction for aquatic organisms under certain stresses, play an important role in bioassessment. Through reference review, field sampling and laboratory analyses and identification, basic environmental data during 1998-2007 and benthic macroinvertebrate data during 2006-2007 were obtained from 23 sampling plots in Dagujia River. Based on environmental gradient calculated by the methods of equivalent standard index and arithmetic mean, as well as the tolerance value for benthic macroinvertebrates estimated by the weighted method, the tolerance value table was established with 44 taxa for benthic macroinvertebrates in Dagujia River. Results showed that (1) it was more objective to estimate tolerance values for benthic macroinvertebrates in Dagujia River by mathematic methods based on the environmental factors and benthic macroinvertebrates than those based on “expert opinions” or environmental gradient based on biodiversity; (2) Dominant benthic macroinvertebrates are sensible and tolerant to environment, and few transitional ones could be observed in Dagujia River; (3) Compared with the results reported in previous references, the tolerance values for some benthic macroinvertebrates in this study were similar in order and family, while they were somewhat dissimilar in genus and species. However, the identification for benthic macroinvertebrates is actually primary, and the substrate and hydrological properties have not been considered as calculating the environmental gradient in this study, thus further researches will be needed to improve the results.