Wheat seeds germination and seedling growth were investigated to study the physiological response of Yanfu 188 and Yangmai 16 to TCB toxicity. The influence of increasing concentrations of TCB （0、0.3、0.6、1.0、1.5 mmol/kg sand and 2.0 mmol/kg sand）on the germination rate and germination exponential of wheat seeds, the biomass of wheat seedling were measured. Soluble protein content, malnodialdehyde (MDA) content, superoxide dismutase (SOD) activities, peroxidase (POD) activities, and catalase (CAT) activities of both leaves and roots were also measured. TCB had obvious toxicity to wheat, and there was significant difference (P<0.05) between Yanfu 188 and Yangmai 16. The seed germination rate, germination exponential and the seedling biomass of Yanfu 188 were higher than those of Yangmai 16. No significant difference (P>0.05) was observed between the control and the treatment on the leaf soluble protein content of Yanfu 188 under TCB stress, but the leaf soluble protein of Yangmai 16 were significantly lower (P<0.05) than the control. With increasing TCB level, the root soluble protein content of Yanfu 188 increased, significantly different with the control. While that of Yangmai 16 increased firstly, then significantly decreased. Under TCB stress, the activities of SOD, POD in Yangmai 16 leaves were lower than control, and the activity of CAT was significantly lower than control. SOD activity in Yanfu 188 leaf was similar with the control. POD and CAT activities in Yanfu 188 leaf was similar with the control under low concentrations, while lower than the control under high concentrations. MDA content in Yanfu 188 was lower than in Yangmai 16. The activities of SOD, POD in Yanfu 188 roots were not sensitive to TCB stress, while those in Yangmai 16 root increased with the concentration of TCB, under induction of O-·2. Since O-·2 producing velocity in Yangmai 16 was greater than Yanfu 188, it could not eliminate excessive O-·2, which led to its higher MDA content. In conclusion, the germination, seedling growth and physiological function of Yanfu 188 were better than Yangmai 16 under TCB stress, and Yanfu 188 was not adversely impacted by TCB.