The objective of this paper is to study the response of the introduced Pinus pinaster Ait. seedlings to the environmental factors. Some of the suitable conditions of seed germination and seedling growth have been considered. The results showed that the suitable conditons which can improve the seed germination percentage during the orthogonal design both in the nursery and laboratory are as followings, the substrate soil, non-mycorrhizae and irrigating once a day are key factors in the laboratory; covered with 1cm soil, irrigating once a day and with the density of 800 stems/m2 are suitable combinations in the nursery. Different rooting powder and concentration treatments caused different seed germination percentage, using GGR 10mg/kg has highest seed germination percentage, the next are GGR 40mg/kg、ABT 40mg/kg and GGR 25mg/kg. There are 4 phases during the Pinus pinaster seedlings growth. In the first and second growing season, the height growth all showed two peaks. As to the total height growth and ground diameter growth, the bare-root seedlings are higher than the container ones; seedlings provenance from Zhejiang province have good performance than those from Shandong province. Also, the seedlings from France growth better than those from Spain; Pinus pinaster has strong salinity tolerance and grows better in the soil with slight acid and higher nutrient content.