The relationship between levels of 19 secondary compounds in rice leaves and resistance of 12 rice varieties resistance to Xanthomonas oryzae pv. oryzae was investigated using high-performance liquid chromatography(HPLC). The relationship between level of X. oryzae resistance amd the area values of chromatographic peaks were significant (R’=0992，p<0.01). A resistant predictive model was established through multiple regression analysis: Y=10.7603 + 01823X1- 0.2287X2 + 0.2163X8 - 2.1975X10 + 0.0728X12 - 0.7438X14 + 1.1484X16 - 0.7795X18. This model indicates that peak 1, peak 2, peak 8, peak 10, peak 12, peak 14, peak 16 and peak 18 are major secondary compounds that may influence varietal resistance to X. oryzae. The resistance of rice varieties to X. oryzae was closely associated with qualitative and quantitative combinations of the secondary compounds, suggesting that X. oryzae resistance arises from the combined action of several secondary compounds that vary in contribution to resistance of the varieties. These secondary compounds may be useful as markers to evaluate and identify the rice varietal resistance to X. oryzae.