The investigation was conducted to detect the inhibition effects of secalonic acid F （SAF） produced by Aspergillus japonicus on higher plant by using gene chip technique, the effects of SAF depended on its concentration，many genes associated with cell death were up-regulated after treated by SAF, the number and expression levels induced by higher SAF concentration were higher than those induced by lower concentration. Genes associated with defense function were also up-regulated by SAF，however，gene number and expression levels induced by higher SAF concentration were lower than those treated by lower concentration. In addition, results showed that SAF acted as auxin，high concentration of SAF might induce the production of ethylene and affact ceramide content which might result in senescence and death in the leaves of receptor plant. The findings brought some new clues for further studying the action mechanism of SAF and non-host selection toxin.