樱桃属(Cerasus)植物根围微生物多样性
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辽宁省教育厅高等学校科学研究A类资助项目(051393);沈阳农业大学青年教师基金资助项目(2005035)


Microbe diversity in the rhizosphere of Cerasus plants
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    摘要:

    研究了樱桃属(Cerasus) 5种植物本溪山樱(Cs)、大青叶(Cp)、马哈利(Cm)、考特(Cap)、早红宝石(Ca)各主要生长期根围微生物数量、种群组成、Shannom-Wiener指数(H)、丰富度(S)、Pielou均匀度指数(J)、Simpson 优势度(D)和优势微生物种群变化动态。从樱桃根围共分离到细菌18属,优势菌属主要为假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)和黄杆菌属(Flavobacterium);放线菌为链霉菌的12个类群,优势类群主要为白色类群(Albosporus)、吸水类群(Hygroscopicus)和黄色类群(Flavus);真菌6属,优势菌属为青霉属(Penicillium)。樱桃根围微生物的多样性指数(H)、丰富度指数(S)、均匀度指数(J) 及优势度指数(D) 随樱桃种类不同而发生变化,细菌的多样性和丰富度指数为Ca>Cs>Cp>Cm>Cap,放线菌为Ca>Cp>Cs>Cm>Cap,真菌为Cm>Ca>Cp>Cs>Cap。各生长期根围微生物种类与数量发生变化,新梢迅速生长期Ca、Cs、Cp和Cm根围微生物种类与数量较多,新梢停长期Cap根围微生物种类与数量较多,落叶期5种樱桃属植物根围微生物种类与数量均较少。

    Abstract:

    The rhizosphere microorganisms play very important roles in nutrient and water metabolism, disease resistance, growth and development of plants. An investigation on microbe diversity in the rhizosphere of Cerasus plants was carried out at the Fruit Experiment Station of Shenyang Agricultural University. The population, population composition, Shannom-Weiener index (H), richness index of genera(S), Pielou evenness index (J) and Simpson index (D) of bacteria, actinomycetes and fungi in the rhizosphere of Cerasus sachalinensis Kom. (Cs)、Cerasus pseudocerasus L.(Cp)、Cerasus mahaleb L.(Cm)、Cerasus avium L.×Cerasus pseudocerasus Kom. (Cap)、Cerasus avium L (Ca) with various growth stages were determined with dilution plate separation and the physiological biochemical characteristic identification methods. This study showed that bacteria isolated belonged to 18 genus, among which bacillus, pseudomonas, flavobacterium were the dominant ones. Albosporus, hygroscopicus and flavus were the main dominant streptomyces in twelve groups of actinomyces. Fungi was composed of 6 genus, and penicillium was the dominate ones. Diversity index (H), richness index of genera(S), evenness index (J) and dominant index (D) of cherry rhizosphere microbe varied with cherry varieties, and diversity index and richness index of genera of bacteria followed a pattern of Ca>Cs>Cp>Cm>Cap, while actionmyces followed a pattern of Ca>Cp>Cs>Cm>Cap, and fungi followed a pattern of Cm>Ca>Cp>Cs>Cap. The rhizosphere microbe composition and population changed with the growth stage, the species number and population of microbes were more at the fast growing stage of shoots in five Cerasus plants, and at withholding growing stage of shoots of Cap, while less at defoliation stage.

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吕德国,于翠,杜国栋,秦嗣军,,李芳东,刘国成.樱桃属(Cerasus)植物根围微生物多样性.生态学报,2008,28(8):3882~3890

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