三种濒危木兰植物幼树光合特性对高温的响应
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广东省热带亚热带植物种质资源库建设资助项目(2005B60301001); 国家自然科学基金资助项目(30770173)


Response of photosynthesis in saplings of three endangered Magnolialia species to high temperature
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    摘要:

    以3种木兰科濒危植物香木莲、大叶木莲及大果木莲为试材,利用LI-6400便携式光合作用测量系统、叶圆片氧电极仪及叶绿素荧光仪,在夏季对其气体交换特性及叶绿素荧光参数等进行了测定。结果表明,3种木兰幼树叶片净光合速率(Pn)的日变化曲线呈双峰型,第1个峰出现在08:00,第2个峰出现在14:00~16:00,12:00有明显的光合“午休”现象。3种木兰植物的最大净光合速率(Pmax)为5.2~7.4μmolm-2s-1 , 光补偿点(LCP)为14~17 μmolm-2s-1 ,光饱和光强(LSP)为500~800μmolm-2s-1 ,大叶木莲的Pmax、LSP和表观量子产额(AQY)显著高于香木莲和大果木莲。随着温度升高,3种木兰植物光合放氧速率先升后降,最适温度为35℃,香木莲的放氧速率显著低于大叶木莲和大果木莲。短期高温胁迫下(45~50℃)3种木兰植物的PSⅡ最大光化学效率(Fv/Fm)均明显降低,初始荧光(F0)升高,大叶木莲的Fv/Fm降幅和F0的升幅均显著低于香木莲和大果木莲。3种木兰植物的光合速率和耐高温能力的顺序为大叶木莲>大果木莲>香木莲。

    Abstract:

    The gas exchange feature and chlorophyll fluorescence parameters in seedlings of three endangered Magnoliatia species (M. aromatica, M. megaphylla and M. grandis.) were determined by LI-6400 portable photosynthesis analysis system (LI-6400, Li-cor USA), leaf disc oxygen electrode (Hansatch, King′s Lynn UK) and chlorophyll fluorometer (Maxi-Imaging-Pam, Walz, Germany) in summer. The results showed that the diurnal variation of net photosynthetic rate in leaves of three plants presented a two-peak curve pattern. The first peak and second peak occurred at about 08:00 and at 14:00-16:00, respectively. An obvious phenomenon of “midday depression of photosynthesis” was observed at noon. Maximum photosynthetic rates (Pmax) were 5.2-7.4μmolm-2s-1, light compensation points (LCP) for photosynthesis were 14-17 μmol m-2s-1, and the light saturation intensities (LSP) were 500-800μmolm-2s-1 in leaves of three Magnoliatia plants. The Pmax, LSP and apparent quantum yield (AQY) of M. megaphylla were significantly higher than that of the other two plants. Under saturated CO2 concentration , the oxygen evolution rate increased firstly with increasing temperature and decreased thereafter. the optimal temperature were around 35℃ in three plants of Magnoliatia. Leaves of M. aromatica showed an obviously lower oxygen evolution rate as compared with that in M. megaphylla and M. grandis, When leaves were suffered to a short term of high temperature treatment (45-50℃), the maximal photochemical efficiency of photosystem Ⅱ (Fv/Fm) decreased obviously, while the minimal fluorescence (F0) increased. The changing extent in M. megaphylla was less than that in M. aromatica and M. grandis. Hence, the sequence of photosynthetic rate and tolerance to high temperature in three Magnoliatia plants from high to low is suggested to be M. megaphylla>M. grandis>M. aromatica.

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李芸瑛,窦新永,彭长连.三种濒危木兰植物幼树光合特性对高温的响应.生态学报,2008,28(8):3789~3797

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