Ecosystem services are a hot spot in modern ecological research, but few studies on reservoir ecosystem services have been conducted. Microclimate regulation and airborne bacteria purification were investigated in Meilin Reservoir and Xili Reservoir, Shenzhen, China. Results showed that reservoir ecosystems may help lower air temperature, increase humidity and purify airborne bacteria. In summer, the mean and maximum air temperatures in Meilin Reservoir and Xili Reservoir were 1.59℃, 2.35℃ and 2.56℃, 4.10℃ lower than those in the urban area, respectively. In winter, compared with the urban area, the mean and maximum relative humility in Meilin Reservoir and Xili Reservoir increased by 6.01%, 11.10% and 7.38%, 7.80%, respectively. The number of airborne bacteria (9 and 10 cfu/plate 5min) in Meilin Reservoir, Xili Reservoir was considerably lower than that (107 cfu/plate 5min) in the urban area. The values of microclimate regulation service in Meilin Reservoir and Xili Reservoir were estimated as 1249.13×104 yuan a-1 and 544183×104 yuan a-1, respectively.