遮荫下高原濒危药用植物川贝母(Fritillaria cirrhosa)光合作用和叶绿素荧光特征
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国家自然科学基金资助项目(30572324);国家发改委高技术产业化专项资助项目(\[2005\]1899)


Effect of shading on photosynthetic characteristics and chlorophyll fluorescence parameters in leaves of Fritillaria cirrhosa
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    摘要:

    测量了川贝母遮荫和全光下叶片光合生理参数和叶绿素荧光参数,探讨了其遮荫下生理生态学指标的变化和强光下的光适应机制,且对光合生理的测量过程给出了全面系统的介绍。川贝母日光合曲线呈双峰变化,遮荫后川贝母光合效率(Pmax)、细胞内CO2浓度(Ci)分别增加了31.1%、10%(p<0.01),有效光反应光强域值(PFD)增加了331.5 \[μmol/(m2·s)\],最大表观量子效率(AQY)和气孔导度(COND)也有所增加,暗呼吸速率(Rd)和叶片蒸汽压亏缺(Vpdl)明显降低,此均有利用光合产物的积累;F′v/F′m(光照下反应中心能量捕获效率)、qP(光化学淬灭)、ETR(电子传递效率)、Psips2(PSⅡ的效率)分别增加了14.7%(p<0.01)、8.8%(p<0.01)、10%(p<0.01)、24.2%(p>0.05),促进了川贝母叶片对光能的利用;Fv/Fm(光化学效率)变化不明显,说明“灯笼花”阶段,全光下川贝母没有受到到明显的胁迫,光合机构未遭到破坏。当自然光强超过光饱和点时,川贝母主要通过提高非化学淬灭,耗散过多吸收的热能以防止光合机构的破坏。当出现极端气候(高温干旱)时,川贝母繁育退化现象(“树儿子”或“灯笼花”阶段退回到“一匹叶”生长阶段)可能是对环境适应的主要方式。另外川贝母叶片狭小、叶片倾角较大降低了强光辐射的有效面积,在形态结构上有利于避免过高光强对叶光合器官的损伤。

    Abstract:

    In order to evaluate ecophysiological factors and strategies of photoprotection, parameters of photosynthetic physiology and chlorophyll fluorescence were measured in leaves of Fritillaria cirrhosa exposed to different light conditions. The process of the same kind of experiment was introduced in detail.Results illustrate the curve of diurnal changes of photosynthesis in two peaks. Under shade conditions maximum photosynthetic rate (Pmax) increased by 31.1%, internal carbon dioxide concentration (Ci), 10% (p<0.01), comparing with in full light.The numerical value between the light saturated point (LSP) and light compensated point (LCP) also increased 331.5 \[μmol/(m2·s)\]. At the same time, the apparent quantum yield (AQY) and stomatal conductance (COND) increased markedly.Dark respiratory rate (Rd) and Vapor pressure deficit (Vpdl) decreased remarkably which benefit the accumulation of photosynthetic products.The efficiency of energy harvesting (F′v/F′m),photochemical quenching of chlorophyll fluorescence (qP),electron transfer rate (ETR) and Psips2 increased by 14.7% (p<0.01), 8.8% (p<0.01), 10%(p<0.01) and 24.2%(p>0.05) respectively, suggesting good use of available light. Under these conditions photochemical efficiency (Fv/Fm) increased slightly, with minimal damage to photosynthetic structures. This suggests that growth of F. cirrhosa is not seriously affected when cultivated under conditions of full light.When natural light intensity is beyond LSP, photodamage of Fritillaria cirrhosa was avoided by increasing levels of non-photochemical quenching (NPQ) which dissipates heat. Plants under extreme environmental conditions or if more than three years old “shuerzi” are intolerant and their? leaves become reduced in size (becoming narrower) so as to resemble those of two year old plants “yipiye”. It is likely that this adaptive strategy reduces leaf surface area and hence reduces possible damage to photosynthetic structures.

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李西文,陈士林*.遮荫下高原濒危药用植物川贝母(Fritillaria cirrhosa)光合作用和叶绿素荧光特征.生态学报,2008,28(7):3438~3447

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