The effects of human-impacted on soil properties in a Pinus caribaea stand were studied in an experiment comparing harvesting litter and control (no harvest) plots, in order to understood the mechanism of soil degradation in human litter removal Pinus caribaea stands and establish basis for sustainable forest management. Results showed that compared with those in undisturbed stand, the mean diameter at breast height (DBH) and mean tree height with litter removal significantly decreased by 21% and 8%, respectively. With litter removal, soil bulk density significantly increased by 14%, whereas capillary porosity, non-capillary porosity, total porosity and capillary water significantly increased by 12%, 37%, 18% and 24%, respectively. There was not significant difference in pH value between the disturbed stand and the undisturbed stand. However, litter removal significantly decreased the contents of soil organic matter, total N, total P, total K, alkalized N, available P, available K and clay by 42%, 46%, 16%, 39%, 20%, 15%, 41% and 27%, respectively. The litter removal plot showed that the numbers of bacteria, fungi and actinomyces was 50%, 19% and 13% lower than the non-litter removal plot with a significant difference in bacterium number between the both. The litter removal significantly resulted in the decrease of the activities of urease, acid phosphatase and catalase. Decrease in litter amount by litter removal caused the decrease of organic matter in soil, which resulted in the decline of soil porosities and water holding capacity, and deterioration of nutrient status, the reduction of microorganism number and enzyme activity, as a result stand growth became slow.