This study assessed the high oil soybean variety Ludou9 in the experimental field of Heze Agricultural Academy during 2005-2006. We determined the effect of waving-canopy cultural type on soybean yield and its constituents, the protein, oil and fatty acid of soybean seeds, and the ecological factors of micro-environment. Compared with the control, the yield of waving-canopy groups increased by 11.76% to 253.65kg•hm-2 and plant height decreased 1.21cm, a decline of 1.46%. In addition, all contents of the oil increased by 1.52%, the content of linoleic acid increased by 1.6%, and the content of linolenic acid and stearic acid decreased by 0.14%and 7.20%, respectively. The differences of plant height and branches between waving-canopy density groups were all significant at p < 0.01, per plant seeds and yield were significant at p < 0.05, and the 100 seeds weight was insignificant. The PAR and CO2 of waving-canopy groups increased 67.68μmol•m-2•s-1 and 8.31μmol•mol-1, respectively. This represents increases by 5.06% and 2.45%, respectively. The humidity of waving-canopy groups increased 0.33mbar, or 4.47%. The temperature of waving-canopy groups increased 186℃, or 0.69%, at low temperature growth stage, while the average temperature decreased 0.17℃, a decline of 051%,at high temperature growth stages. The changes of PAR, CO2, temperature and humidity of the micro-environment favoured increased yield and improved quality of the high oil soybean.