The accumulation of Cu in different parts of H. ramosissimum, distribution of Cu in root subcellulars and chemical forma of Cu in its body were studied by means of analysis of field samples and greenhouse cultivation. The results showed that most of the Cu of H. ramosissimum growing on the Cu mining spoils and greenhouse cultivation were deposited in the root with the largest content of 1309 mg•kg-1 and 1339.75 mg•kg-1.The average retention rate was 84.98% and 973%, respectively. Moreover, more Cu deposited in the cell wall than that of the other fractions which accounted for 45%-70% in the root subcelluar of H. ramosissimum. The Cu content distributed in the cell wall (F1 ) and plasma part with ribosome (F4) has a marked correlation with Cu concentration of the soil, respectively (rF1＝0.98，rF4＝0.98，p<001). Under Cu stress of different levels, the chemical forma of Cu in the plant was different. The content of Cu forma extracted by HAC and HCl in the root was the greatest with the rate of 60%-80%, which was significantly and positively related to Cu concentration of the soil, respectively (rHAC=0.997，rHCl=0.98，p<0.01) . Meanwhile, NaCl in the shoot was dominant, while that extracted by water was the lowest in both the root and the shoot. The accumulation character and detoxification mechanism in which the mass of Cu were intercepted at the root accumulated in cytoderms and formed certain low dissolvable Cu-compounds, possibly contributing to the reduction of the Cu toxic effect on H. ramosissimum. It was suggested that H. ramosissimum had a remarkable tolerance to Cu and a potential capacity of Cu accumulation could be used widely in phytoremediation of Cu-polluted soils.