The Locusta migratoria tibetensis is an important pest in the Qing-Tibet Plateau. In recent years, it causes serious damage in Tibet, Yushu of Qinghai, plateau of western Sichuan. The super-cooling points of Locusta migratoria tibetensis were determined. The physiological and biochemical indexes related with the super-cooling capability were also determined. The results indicated the super-cooling point and the freezing point of the egg was the lowest, which was -22.02℃ and -16.36℃ respectively. The nmphs supercooling points increase with increase of hepatin，fat and glycerin content from 1 to 4 instar stage, which was -9.28℃、-8.51℃、-7.18℃、 -6.46℃, respectively. However, the super-cooling point descends to -8.07℃ at 5th instar stage, when the contents of fat, hepatin and glycerin were highest . The correlation between the contents of fat, hepatin and glycerin during different development stage and the super-cooling capacity in vivo was also discussed in detail.