One mutation and two different haplotypes were found when the fifth exon of GH gene sequences of 16 Leiqiong cattle were analyzed. Together with two homologous sequences of Bayingole yak and sequences of zebu (Bos indicus), Bos taurus and Bos grunniens and with the sequence of Bubalus bubalis as the outgroup, the fifth exon of GH gene sequences of 16 Leiqiong cattle were aligned and the base composition and nucleotide variation of GH gene were analyzed. The phylogenetic trees were constructed by the NJ method and the MP method respectively, both supporting almost the same topology.The results indicated that GH gene differentiation happened before Bubalus bubalis differentiated from Bos indicus, Bos taurus and Bos grunniens. Mutiple haplotypes existed in breeds among zebu, conforming that the fifth exon of GH gene variation rate was high.