An experiment was conducted that Glomus mosseea, Glomus versiforme and Glomus.diaphanum were inoculated to Broussonetia papyrifera seedlings with single inoculation, co-inoculation and non-inoculation. Three months later, concentrations of nitrogen and phosphorus and soil enzyme activities seedlings were measured. The results showed that nitrogen concentration of host plants was increased significantly in root, stem and leaf comparing with none-inoculated, the order was rootstem<leaf. Activities of four kinds of soil enzymes (Polyphenol oxidase, Protease, Alkaline phosphatase, Peroxide hydrogenase) increased, and activities of Protease and Alkaline phosphatase were significant difference between inoculation and none disposals. A significant correlation was found between nitrogen concentration with Protease and Alkaline phosphatase, phosphorus concentration was correlated with Polyphenol oxidase. We conclude treatments of inoculation on AM fungus increase contents of N and P host plant with biomass and soil enzyme activites.