Community characteristics of soil sarcodinids and flagellates in the Scenic Spots and Historic Sites of Maijishan, Gansu were studied by using “non-flooded petri dish method” and observation in vivo. Ninety species were totally identified, including 5 unnamed species and 29 new records in China, which belongs to 2 subphylums, 5 classes, 14 orders, 27 families and 46 genera. Results demonstrated the following features: there are abundant species in these areas, including a large number of endemic and rare forms; the community structures are complex and the species composition is unique, and the species distribution was highly heterogenetic. Compared with that of aquatic community, the species composition of soil community of sarcodinids and flagellates in the Scenic Spots and Historic Sites is quite dissimilar, which indicates that the soil and aquatic communities belong to two independent ones.