Incorrect or excessive use of veterinary medicines in recent decades has caused substantial amounts of drugs and their metabolites being released to the environment through manure application onto agricultural land. One of the potential adverse effects is to harm some non-target organisms when they consume the excrement that contains drugs or their metabolites. The purpose of this study is to investigate the effect of Albendazole on the activities of three enzymes in the earthworm, as a whole and at different sections of the body. Earthworms were exposed to different concentrations, 0, 100, 200, 400 and 600 mg/kg. After 2, 7 and 14 days of exposure, acid phosphatase (AP), glutathione S-transferase (GST) and adenosine triphosphatease (Ca²⁺-ATPase) activities were analyzed. The results showed that Albendzole had an effect on all three enzymatic activities. The impacts on AP and GST activities were more significant than that on Ca²⁺-ATPase activitity. The inhibition on AP and GST activities became stronger with the longer exposure. The concentration duration interaction effects were observed for these two enzymatic activities. In addition, for AP activity, there was also a significant interaction effect between concentration and body section. When Albendazole concentration increased, AP activity in the anterior section of the earthworm decreased more significantly compared to the whole earthworms and the other two sections. In the anterior section, AP activity was still reduced to 78% of the control value at 100 mg/kg after 14 days. AP activity in the mid-part, after 14 days, changed from 84% to 63% of the control value when the concentration increased from 200 mg/kg to 600 mg/kg. The degree of AP activity change with the concentration was even less for the whole earthworm and the posterior section. For Ca²⁺-ATPase activity, the most significant inhibition was observed in the mid-part after 7 days. The activity was reduced to 63%-49% of the control value when the concentration was from 200 mg/kg to 600 mg/kg. There were no significant concentration section and concentration duration interaction effects for Ca²⁺-ATPase activity.