Parthenocissus tricuspidata Planch is a typical woody vine plant in subtropical area. It is planted widely for vertical surfaces landscaping, soil erosion control, and revegetation. However, little is known about the relationship between nutrient supply and Parthenocissus tricuspidata Planch growth. A hydroponic culture experiment was conducted to investigatethe effects of different nitrogen rates （0, 0.15, 0.30, 0.45, 0.60, 0.75 g•L^-1） on Parthenocissus tricuspidata Planch seedling growth, nutrient use efficiency, and nutrient distribution in different plant parts. Supplying high nitrogen to Parthenocissus tricuspidata Planch significantly increased plant biomass. Plant biomass allocation ratios among root, stem, and leaf were also affected by different nitrogen rates. For treatments supplied with nitrogen, leaf accounted for more than 50% of total plant biomass, while stem and root accounted for less than 40% and 10%, respectively. Increasing nitrogen rate increased nitrogen content in root, leaf, and stem, but did not have significant effect on phosphorus content. Supplying nitrogen to Parthenocissus tricuspidata Planch reduced potassium content in stem and leaf because of dilution effect, but the trend with increasing nitrogen rate was not obvious. Leaf was the main tissue for nitrogen accumulation, accounting for more than 60% of total accumulated nitrogen in plant. Higher nitrogen rates reduced nitrogen use efficiency, but increased phosphorus and potassium use efficiency by Parthenocissus tricuspidata Planch. The results indicated that nitrogen nutrition could significantly influence Parthenocissus tricuspidata Planch growth, nutrient use efficiency, and nutrient distribution in plant tissues.