The differences in absorption and accumulation of heavy metals were investigated in various plants and their organs, such as Pinus tabulaeformis and Populus simonii, along the highway in western Inner Mongolia. The result showed that the highest uptake to heavy metals and pollution degree in surface soils of plant is Cd. With the atomic number increased (Cr to Pb), the heavy metals concentration displayed "N" figure in the roots, stem-leaf of Pinus tabulaeformis and Populus simonii. Furthermore, the heavy metals concentration in different organs of different plants followed the order of Zn > Cu > Ni, Cr, As, Pb > Cd > Hg. A higher enrichment capacity was observed in stem-leaf of Populus simonii for Cr, Ni and Pb compared with its root whereas there was a reverse trend in stem-leaf of Pinus tabulaeformis. The levels of heavy metals in various plants were independent on total content of theirs with available phases, but dependent on their proportions. The comparison between the concentration of availability phases in the soils and the concentration of heavy metals in plants shows the same sequence. That is the sequence of percent of the availability phases concentration to the total concentration dissimilar the sequence of the concentrations of heavy metals in the different organs in the different plants.