CO₂ enrichment stimulates the rate of photosynthesis and growth, plants mass is also enhanced under CO₂ enrichment. Few studies have addressed the photosynthesis and growth of Anthurium andraeanum L. with higher ornamental value under CO2enrichment. Therefore, the effects of elevation of CO₂ for 150d on net photosynthetic rate, growth rate and photosynthetic enzymatic activity of Anthurium andraeanum L. seedlings were studied in open-top plastic chambers. The results showed that plants grown under T1 treatment ((700±100) mol mol^-1 CO₂) for 90 d, the plant height,leaf area and fresh weight increased by 15.76%, 14.30% and 29.622% respectively in comparison with those of plants grown under ambient [CO2]( CK (360±30) mol mol^-1 CO₂). There are 15.00%, 9.63%, 36.22％increment respectively for T2 treatment((1000±100)μmol•mol^-1 CO₂); At the treatment of 150 d, the plant height,leaf area and fresh weight of T1 increased by 16.08%, 17.30% and 49.09% respectively in comparison with those of CK, there are 16.61%, 10.10%, 48.87％increment respectively for T2 treatment; However, plants for T1,T2 treatment under individual growth conditions at the whole period, their net photosynthetic rate were higher than those grown under the ambient [CO₂], at the treatment of 150 d, Pn in T1, T2 increased by 8.25%,20.62% in comparison with that in CK. Photosynthetic acclimation might start to appear in that the Pn in T1, T2 plants determined under ambient [CO2] were lower than that for CK from 90 d. Elevated [CO2] caused a marked rise in soluble sugar and starch accumulation in leaves, but significantly reduced their stomata conductance and transpiratory rate; The starch content in T1,T2 was 52.60%, 67.66% higher than that in CK at the treatment of 90 d; The starch and sugar content in T1 increased by 53.43%, 6.32% respectively in comparison with those in CK at the treatment of 150 d, there were 58.44%, 8.07% increment for T2, the chlorophyll content in T1,T2 presented lower in comparison with that of CK from 90 d. In addition, elevated [CO2] had positive effect on Rubisco activity firstly and negative effect from 90 d, and glycolate oxidase activity apparently decreased in leaves through the whole experiments, the glycolate oxidase activity in T1, T2 decreased by 41.28%, 45.35% respectively at the treatment of 150 d. Increase in CO₂ concentration in a certain extent (90 d) might be most beneficial for increasing net photosynthetic rate and the content of carbohydrate accumulation, and furthermore, the vegetative growth, but the promotion decreased slowly if CO₂ enrichment prolonged.