Agricultural land provides not only food and fiber, but also different non-market commodities, with characteristics of externalities or public goods. For example, Agricultural land provides a variety of important environmental and social services including climate adjust, wildlife habitat, waste treatment, recreational opportunities, food security and so on. Because most of these services are public goods, the non-market value of agricultural land preservation cannot be directly obtained from the market. So estimating the non-market value of agricultural land is one of the focuses in the field of resources and environmental economics. Nowadays, it is very important to evaluate the non-market value of agricultural land resource, because it can not only provide the scientific basis for rural-urban land conversion decision-making, but also decrease the risk of high quality agricultural land loss. In this paper, the authors use the contingent valuation method (CVM) to evaluate the non-market value of agricultural land in Wuhan according to 471 valid questionnaires. Several conclusions can be drawn. Firstly, it shows that the residents (including farmers and urban citizens) have recognized the non-market values of agricultural land base with the increase in awareness of agricultural land protection. According to the survey, recently 83.65% of the respondents have positive WTP to the protection of agricultural lands. Secondly, according to the residents `willingness to pay for protecting local agricultural lands, the whole non-market value of agricultural land reaches 30.0035×108 RMB￥ (US3.7504×108), cultivated land’s non-market value is almost 116154 RMB￥ (US14519) per hectare in Wuhan. And the non-market value of different ecotype agricultural land is correlated with the endowment of local agricultural land resources. The more abundant the agricultural land resource, the lower the non-market value, and the vice versa. Different agricultural land has different non-market value. The non-market value of cultivated land is 59171 RMB￥ per hectare (US7396/hm2), the proportion of existence value, option value and bequest value is 34.59%, 33.27% and 32.14%. The non-market value of horticultural land is 955543 RMB￥ per hectare (US119443/hm2), the non-market value of forestry land and water are 204078 RMB￥ (US25510) and 145711 RMB￥ (18214) per hectare respectively. Thirdly, farmers play an important role in agricultural land preservation in our country, so if we estimate the non-market value basing on farmers′ willingness to accept, cultivated land′ s non-market value is almost 16447.57 RMB￥ per hectare (US2055.95/hm²). Finally, if the environment of agricultural land is disappearing, citizen will want to accept the compensation from government. From this point, average household’s willingness to accept is 8612 RMB￥•a-1 (US1076.5•a^-1), the total non-market value of agricultural land is 109.5685×108 RMB￥ (US13.6961×10⁸) every year, and the agricultural land′s non-market value is almost 424200 RMB￥ per hectare (US53025/hm²).