Roads, especially large highways and railways can have a significant impact on wildlife movement and survival. These roads not only separate the previously connected areas of habitat, but they also create barriers that cumbersome wildlife movement between patches, particularly for large mammals, such as ungulates. Some of these impacts could be mitigated if wildlife can find and utilize passageways under highways and railways. The effect of traffic and railway buildings on wild animal activities during day time along Qinghai-Tibetan highway between Budongquan（35°17′N; 93°16′E） to Wudaliang（35°13′N; 93°04′E） was studied in August 2003 and August 2004. Furthermore, passageways under Qinghai-Tibetan railway were monitored to determine association between probability and frequency of passageways and the distance to Qinghai-Tibetan highway and passageways dimension variables. The result showed that, the traffic during daytime had some effects on Tibetan antelope (Pantholops hodgsoni), Tibetan gazelle (Procapra picticaudata), Kyang (Equus kiang) when they were passing the road, especially significant for Tibetan antelope. At the same time, they could habituate themselves to the changes of surroundings by learning and adjusting their behavior. Most of their activity took place in the morning in order to avoid the impacts result of traffic, and they also could find and utilize passageways under Qinghai-Tibetan railway. We found that the likelihood of utilization were significantly positive correlated with the distance to Qinghai-Tibetan highway. Passageways dimensions, surrounding habitat and human activities could influence themselves utilized by wild animal. Most passageways under Qinghai-Tibetan railway could not be effectively utilized by wildlife for their low length, low height or human activities. Anyway, wildlife could habituate themselves to the changes of surroundings owing to the Qinghai -Tibetan railway building by learning and adjusting their behavior.