The green lacewing,Chrysoperla sinica (Tjeder),a common species in China,is known as an important natural enemy attacking many kinds of agricultural and forestry pests.It is one of the important dominant natural enemies in Tai’an of Shandong Province.Under natural conditions, it has four generations and obvious metagenesis during the whole year,and overwinters in adults of the fourth generation in facultative diapause.In the present study, experiments were carried out to examine the capability of the adult cold hardiness among the different generations,especially the seasonal changes of the water and fat content in adult body and the supercooling point (SCP) were determined.Results showed that the cold hardiness of the female and male adults took on seasonal changes that the cold hardiness gradually strengthened with the winter temperature decreasing and weakened with the temperature increasing after winter.The water and fat content,the SCP and freezing point (FP) of the female and male adults changed greatly among the generations in the different seasons.The water content and SCP of the overwintering female and male adults during the pre- and mid-overwintering period were significantly lower than that of other generations in growing seasons,but the water content and SCP had no difference between the adults during the late overwintering period and other generations in growing seasons.The regression analysis showed that there was remarkably negative correlation between the water content and SCP of the adults (p<0.01).The total fat content of the overwintering generation (especially at the pre-overwintering period) was significantly higher than that of other generations,and the correlation between the total fat content and SCP was significantly positive in female adults (p<0.05),otherwise,there was no correlation in male adults(p>0.05).Compared the female adults with the male adults in the different generations,the water content had significant difference only in the pre-overwintering period,and the SCP and the total fat content had significant difference only in the late overwintering period.