Abstract:he seabuckthorn carpenter moth, Holcocerus hippophaecolus Hua, Chou, Fang et Chen is recently a major pest of seabuckthorn (Hippophae rhamnoides) in Inner Mongolia Autonomous region, Liaoning, Shanxi, Ningxia and Shaanxi provinces in China. The generation time of H. hippophaecolus is four years. The larvae cause damage primarily to the stems and roots of seabuckthorn, and the mature larvae pupate in soil. Spatial distribution regularities were analyzed using biostatistics and geostatistical methods for the carpenter moth pupae in order to effectively control the insect and to further study the spatial distribution of the population. The results showed that the eclosion time of most of the pupae (90%) is from early June to the end of July. The sex ratio of the pupae was nearly 1:1 in the woodland samples. 24.3% of the trees were found to have pupae, ranging from 0 to 4 pupae per tree. The pupae were found within a distance of 1.3 m from the base of the stem, but 90% of the pupae were found to be aggregated within the distance of 1 m from the base of the stem. The pupae showed intense spatial aggregation in the studied woodland, 11.1m spatial dependence and 90.7% intensity of local spatial continuity were found, the population presented intensive spotted distribution, and many aggregated spots were found in the whole woodland. In the study on the relationship between different grid sizes and variogram of the pupae, the variation in the range, the intensity of local spatial continuity and the sill was very low or non-existent when the grid size was 5m, 6m or 7m, the value of the decisive coefficient was more bigger when the grid size was 5m, so, it is ideal grid size.