The planet is experiencing a global climate changing, global warming affects distribution, penology and breeding of wildlife. Metrological records indicate that the Qinghai Lake drainage area is also experiencing such a climate change. Land-cover in the region is also changed. From 2003 to 2005, we investigated wild vertebrate fauna in the Qinghai Lake region under the environment change. With reference to literature, using remote sensing images, GIS methods and field surveys, we found that climate and environment changes in the Qinghai Lake region are profound. Annual average temperature in the Qinghai Lake region increased, especially those in autumn and winter.Compare with that of the 1980s, the average temperature in this region increased 1.26℃, whereas precipitation in the Qinghai Lake region decreased and aridity increased slowly at the end of the 20th century. Water table level in the Qinghai Lake descended 3.7m and the area of Qinghai Lake decreased 313.3 km² since 1959. Since 1976, the desert area around the lake has increased from 356.4 km2 to 735.9 km2, while the swamp area has decreased from 25.08km2 to 4.73 km². From 1949, human population has increased about ten times in Qinghai Lake region. A transportation system of road, provincial high, national highway and railway has been established. A large area of grassland was fenced. In a word, living environment for wild vertebrates in Qinghai Lake deteriorated under the influence of global change. Suitable habitats for frogs, toads and waterfowls decreased and the swamps disappeared. Deserts on the lakeshore expanded as the water table level in the lake decreased; the habitat area of hamphead lizard increased. We recorded 46 birds and 10 mammals in wetlands, 3 birds and 19 mammals in deserts, 63 birds and 36 mammals in alpine meadow habitat, 16 birds and 6 mammals in the human settlements. Different habitats are inhabited by different rodents. Alpine meadow ecosystem has the most abundant rodent species whereas the desert ecosystem has the least. Some birds and mammals such as the Tibetan antelope, Tibetan wild ass and wild yak have disappeared from the Qinghai Lake region while the density of Przewalski’s gazelle, Tibetan gazelle, musk deer and red deer decreased remarkably.