Relative water content (RWC), operation and development of alternative pathway, the changes in active oxygen species (AOS) and activities of antioxidant enzymes, and the role of alternative pathway participating in the protection mechanism against environment stress were studied and discussed in wheat seedlings leaves under combined intimidation of water stress and pathogen infection. Experiments showed that the disease-resistant cultivars had more powerful ability to regulate moisture content than the disease-susceptible cultivars under combined intimidation of water stress and pathogen infection. The effects of stripe rust on cyanide-resistant respiration were very larger than that of water stress. The decrease in cyanide-resistant respiration caused by water stress couldn’t counteract the increasing cyanide-resistant respiration aroused by stripe rust infecting. Combined intimidation resulted in AOS accumulation, and cyanide-resistant respiration and the activities of antioxidant enzymes presented upon the completion of lowing AOS production, to some extent. It was proposed that cyanide-resistant respiration participates in the anti-oxidation mechanisms and regulate the ambivalence and balance of requirement of the energy and substance for combined intimidation conditions.