The spider Latrodectus tredecimguttatus which was originally found in Xinjiang Province has been successfully man-mu/tiplied in the south of china. This report described the methods of feeding. Including the foods and the rearing chest and the observations of the effect of humidity and temperature to the living rate and the ecdysis rate of this speacies of spider. We found that the optimum temperature and humidity for the living of this spider were 25℃ and 65%, respectively, and the optimal food is fly. With the combination of the indoor and field investigation, the relationship of the biotope and the animal density of this spider was analyzed.