外源一氧化氮提高一年生黑麦草抗冷性机制
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Q948

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Studies on mechanism of enhancing the chilling resistance of annual ryegrass by exogenous nitric oxide
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    摘要:

    用不同浓度的一氧化氮(NO)供体硝普纳(sodiumnitroprusside,SNP)处理低温胁迫下1年生黑麦草幼苗,探讨外源NO对提高黑麦草幼苗抗冷性的作用。结果表明外源NO能减缓低温胁迫下黑麦草幼苗质膜相对透性的增加,促进脯氨酸(Pro)的积累,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)保护酶活性,其中POD酶活性的提高尤为显著。恢复生长时,经SNP处理的幼苗膜透性、脯氨酸和保护酶活性恢复较快,其中0.5mmol/LSNP处理的效果最为明显,0.2、1.0mmol/LSNP处理的效果次之。

    Abstract:

    The annual ryegrass (Lolioum multiflorum) was used in this study. The seeds of ryegrass were germinated in a growth chamber at 20±1℃, with 60% to 80% of humidity, under 12h light (400 lx)/12h dark. The continuous illumination was provided by cool-white fluorescent lamps (400 lx). When the second or the fourth leaves fully expanded the normal leaves were selected for the experiments. SNP ([Na_2Fe(CN)_5]·NO, Merck, Karmstadt, Germany) was used as NO donor. In the experiments the stock solution of 10mmol/L SNP...

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马向丽,魏小红,龙瑞军,崔文娟,万引琳.外源一氧化氮提高一年生黑麦草抗冷性机制.生态学报,2005,25(6):1269~1274

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