Application of polymerase chain reaction-denaturing gradient gel electro-(phoresis) (PCR-DGGE) to the analysis of changes of microbial ecological communities in activated sludge systems
Population dynamics in two activated sludge systems inoculated with seed sludge cultivated respectively at a low temperature ((5±1)℃) and normal temperature ((20±1)℃) were followed under the same operation conditions using the PCR-DGGE method. The genomic DNA was extracted from activated sludge using phenol-chloroform. PCR amplification of the extracted microbial 16S rRNA gene fragments was performed using primers GC341F and 518R. The result of agarose gel (1.8%) electrophoresis shows that the PCR product...
