The culturing condition for isolating ecotomycorrhizal fungus Tricholoma spp. was performed, and 10 matsutake isolates were got from Yajiang, Sichuan Province. Using NS1 and NS6, NS1 and NS8, ITS4 and ITS5 primer pairs, the 18S rDNA and ITS fragments of these matsutake strains was amplified, and digested by AluI, HaeIII, HinfI and MspI, respectively. The results showed that these strains had the same restriction patterns for each restriction enzyme, which showed that the homology among these strains were ve...