In plants, freezing tolerance increases upon exposure to low temperatures, which is known as cold acclimation. Cold acclimation in plants is a complex process involving physiological, biochemical and energetic changes. These include membrane stabilization, the accumulation of soluble proteins, and small penetrable molecules such as proline, sugars and etc. Some of these changes are essential for plants to survive freezing temperature, whereas the others are not. Recent advances in understanding the physiolo...