Green infrastructure can provide efficient and accessible flood regulation ecosystem services; the measurement of supply and demand is the basis and prerequisite for improving ecosystem services and can provide decision-making information for land use planning and green infrastructure planning. This paper elaborates on the flood regulation ecosystem services of green infrastructure, including the conceptual connotation, main body composition, and relationship for each scale of supply and demand. Various methods of measurement for flood regulation service supply and demand are reviewed; the methods for supply measurement include land use method, ecosystem methods, and the flood storage model, while the methods for demand include the risk assessment method, flood submerged model, and economic loss statistics. Based on the existing research on the supply and demand of ecosystem services, we envisage a supply and demand analysis framework for flood regulation services. Through quantity balance and space matching for supply and demand, the relationship of supply and demand is divided into four scenarios. For each scenario, there are three regulation strategies:regulation for supply and demand toward dynamic balance, adjustment of supply and demand for quantity match, and service flow mechanism for the spatial match. Finally, we summarize deficiencies in the current research and propose future research directions with the aim of enlightening research on green infrastructure planning and ecosystem service management and decision-making.