As the top of the wetland food chain, red-crowned cranes is an indicator species of wetland quality. At present, the declining population of red-crowned cranes has drawn widespread attentions of wildlife protection people. Habitat loss and fragmentation may be the main reasons of the population declination of red-crowned cranes. When the research is going on, it was suggested that multi-scale characteristics about habit can help us to advance knowledge of bird selection. Yancheng nature reserve in Jiangsu province is the most important overwintering habitat for the west population line of red-crowned crane in the world. It is of great practical significance by taking Yancheng as the research area for the protection of red-crowned cranes. Remote sensing images was used to identify the landscape of study area, and four field observations were processed to investigate the number and the distribution of red-crowned cranes population during the winters of 2015-2016. The method of polar coordinates in GIS was used to map the foraging distribution of red-crowned cranes. The multi-scales, which contained point buffer scale, landscape scale and region scale, were selected to analyse the utilization of the overwintering habitat of red-crowned cranes. On the point of buffer scale, the buffer and overlay analysis of GIS were achieved by using ArcGIS 10.2 software. This method can describe the habitat use intensity of red-crowned crane. The results showed that red-crowned cranes like stay in the vegetation that covered with Suaeda best, and reed better. The coverage and the height of vegetation which were less than 30% and 60 cm respectively are well received by the red-crowned crane. On the landscape scale, field surveies of population numbers in multi-landscapes were recombined into single landscape. The results showed that the top three preference habitats of red-crowned crane were reed marshes, Suaeda marsh, and water. Suaeda marsh, reed marsh and water were selected by the red-crowned crane. They did not select Spartina alterniflora marsh as their habit. On the regional scale, the interference intensity was described by road and land use. The response of the red-crowned crane to the interference was discussed by the neighborhood analysis. The red-crowned cranes were distributed in the core area of the low interference with large area. In addition to interference, the habitat area and its fragmentation are closely related to the habitat selection of red-crowned cranes. The landscape index of different interference intensity showed that the low disturbing habitat with less than 2 km² and higher fragmentation will not be used by red-crowned cranes. Based on the result of three scales, we concluded that the habitat selection of red-crowned crane has obvious multi-scale utilization characteristics. Multi-scale habitat characteristics should be considered while protecting red-crowned cranes.