Intercropping, with 1000-year history in Chinese agriculture, is still widespread in modern Chinese agriculture. The environmental resources, such as light, water and mineral nutrients, might be used more efficiently in intercropping than in monoculture by crops, which resulted in greater yield. The interaction among crops in intercropping might help the usage of environmental resources by crops. One of the vital roles of legumes played in cropping systems is their ability to fix nitrogen from air. However, Species of Leguminosae are known as weaker competitors to species of Gramineae. Theconcentration of soil inorganic N following legumes was often higher than that following cereal crops. To study the effects of maize-soybean intercropping and nitrogen levels on corn yield, main agronomic characters and growth dynamic, we conducted a double-factor randomized block experiment of two planting patterns (corn monoculture and maize-soybean intercropping) and two nitrogen levels (0 kg/hm², 150 kg/hm²). The results showed that: cropping patterns and nitrogen levels had no significant effect on the yield of spring maize; the yield of monoculture maize in autumn with N application (150 kg/hm²) was significantly higher than the other three treatments. Comparing to no nitrogen treatment, hundred-grain weight with N 150 kg/hm² increased by 18.92% and 19.23% respectively in the mode of spring maize monoculture and intercropping. In the mode of autumn maize monoculture and intercropping, hundred-grain weight of the treatment with N 150 kg/hm² was significant higher than those of no nitrogen treatments, increased by 31.03% and 32.75% respectively. There was no significant difference in maize hundred-grain weight between intercropping and monoculture under the same N application. There was no significant difference of ear length among the treatments of spring maize. Comparing to no nitrogen treatment, the ear length of autumn maize in the treatment with N 150 kg/hm² in monoculture and intercropping patterns were both significantly improved. On the aspect of ear width, there was no significant difference between the treatments of spring maize; whilte autumn maize monoculture increased by 18.67% under N 150 kg/hm² comparing to N 0 kg/hm². However, intercropping had no significant difference in autumn maize. On the aspect of spike grain number, there was no significant difference between the treatments of spring maize; while autumn maize grain number was significantly higher than the other three under monoculture and N 150 kg/hm². Nitrogen and intercropping both could improve SPAD value and plant height of maize, and promote the maize dry matter accumulation, while there was no significant difference among the treatments. Overall, cropping patterns and nitrogen levels had effects on maize yield, main agronomic characters and growth dynamic. The effects of intercropping were less than nitrogen level, because soil and Leguminosae had a certain capacity of supplying nitrogen for maize. The effects of nitrogen application on spring maize were only presented in the hundred-grain weight. As the nitrogen in soil was absorbed by maize, the nitrogen supporting from soil decreased, leading to the significantly effects of nitrogen application in the autumn maize.